The attack is one of a few Gingrey's campaign lobs at his opponents in the ad, flagged by Slate's Dave Weigel on Monday. With Handel, the attack refers to her voting in support of funding for an LGBT group.
"Georgians can send a true conservative to the Senate but will we condone Karen Handel's vote for youth pride that supports homosexuality?" the narrator in the ad said.
The attack refers to a vote Handel cast as commissioner of Fulton County in 2006 to fund the LGBT Youth Pride group.
The ad then goes on to attack frontrunner David Perdue on Common Core and Rep. Jack Kingston (R-GA) for "surrendering" on Obamacare.
The ad is a last ditch attempt by Gingrey, who looks poised to lose the primary on Tuesday. Watch the ad below: