During the opening monologue of the ceremony, Poehler said that the movie "12 Years a Slave" changed how she viewed slavery, prompting a puzzled reaction from co-host Tina Fey.
"I loved '12 Years a Slave' and I can honestly say that after seeing that film, I will never look at slavery the same way again," Poehler said.
"Wait, how were you—" Fey began to ask before Poehler quickly moved on to the next joke, drawing laughter from the audience.
The gag harkened back to Cohen's infamous reaction to "12 Years a Slave" that he detailed last year in his column for the Washington Post.
In a piece published in November, Cohen wrote that Steve McQueen's critically acclaimed film opened his eyes and showed him that "slavery was not a benign institution in which mostly benevolent whites owned innocent and grateful blacks."