The 60-second ad, titled "Captain Khan," features Khan fighting back tears and comforting his wife, Ghazala, as he somberly explains the details of his son's death. Later in the video, footage of his son's funeral at Arlington National Cemetery plays as Khan addresses a question to Trump.
“I want to ask Mr. Trump, would my son have a place in your America?" he asks after an image of Trump comes on the screen.
The Clinton campaign said the ad is expected to air in the battleground states of Florida, Iowa, Nevada, New Hampshire, North Carolina, Ohio and Pennsylvania and is part of an already existing ad buy.
Khan and Trump engaged in a very public battle after Khan's speech at the convention, where he held up a pocket constitution and asked if Trump had read it. When Trump fought back, questioning if Khan forbid his wife from speaking onstage, many Republicans were horrified and disavowed his attacks on a Gold Star family.
Watch the ad below: