Attorney General Jeff Landry filed the court challenge Thursday, saying Gov. John Bel Edwards' anti-discrimination order violates state law and exceeds Edwards' authority.
The two men have clashed repeatedly over the order, which prohibits discrimination in government and most state contracts based on sexual orientation and gender identity.
Landry has blocked dozens of contracts to let state agencies hire outside lawyers that contain the anti-discrimination clause.
Edwards refuses to rescind the order and has sued Landry for his refusal to approve the contracts.
A judge ruled against Edwards this week on a technicality, not on the substance of whether the governor's executive order is legal.
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